BTK Knockout cell line (U-2932)

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location： Home > Products > KO Cell Lines > BTK Knockout cell line (U-2932)

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BTK Knockout cell line (U-2932) Order

BTK Knockout cell line (U-2932)
Catalog#
YKO-H067
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BTK Knockout cell line (U-2932)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
BTK Knockout cell line (U-2932)
Cell Morphology
Human Lymphocyte-like, suspension
Passage ratio
1：2-1：4
Species
Human
Gene Name
BTK
Gene ID
695
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_E1E7(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript BTK-201)
Validation: PCR+Sanger Sequencing
BTK Knockout cell line (U-2932) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:U-2932		 STR Info (Cell bank)
Cell Line:U-2932
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11111111
D7S820 913913
D16S539 12121212
VWA 14171417
TH01 9.39.39.39.3
AMEL XXXX
TPOX 11121112
CSF1PO 10121012
D12S391 1820
FGA 2224
D2S1338 2223
D21S11 2830
D18S51 1515
D8S1179 1314
D3S1358 1516
D6S1043 1818
PENTAE 1314
D19S433 1414
PENTAD 1414
D1S1656 1616.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BTK gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-2932 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BTK gene knockout BTK Knockout cell line (U-2932) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
BTK Knockout cell line (U-2932)
BTK Knockout cell line (U-2932)
Catalog#
YKO-H067
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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